Learn vocabulary, terms, and more with flashcards, games, and other study tools. The development of biomaterials for tissue engineering applications is continuously improving, accordingly to the needs to generate an ideal cellextracellular matrix interaction. Extracellularmatrix tethering regulates stemcell fate. Ungricht and kutay, 2017, tether dna to the lamins, thus creating a bridge. Integrinbased adhesomes transmit physical and chemical signals between cells and the extracellular matrix. Extracellular matrix as a regulator of epidermal stem cell. Role of the extracellular matrix in regulating stem cell fate.
Although the matrix rigidity and elasticity were not tested in. Frontiers selfassembling peptides as extracellular matrix. Hadden1 and yu suk choi2 abstract each cell forever interacts with its extracellular matrix ecm. The extracellular microscape governs mesenchymal stem cell. Recent studies have made significant progress in understanding stem cell ecm interactions. Interplay of matrix stiffness and protein tethering in stem. In epidermis, ecm is represented, primarily, by a highly. Extracellularmatrix tethering regulates stemcell fate b trappmann, je gautrot, jt connelly, dgt strange, y li, ml oyen. Stemcell fate is known to be regulated by signals from the microenvironment, one of the environmental parameters being the extracellular matrix ecm to which stem cells adhere 1. The stiffness, nanotopography, protein composition, stress and strain inherent to any given ecm. This item appears in the following collections faculty of science 27228. Trappmann b1, gautrot je, connelly jt, strange dg, li y, oyen ml, cohen stuart ma, boehm h, li b, vogel v, spatz jp, watt fm, huck wt.
The ecm extracellular matrix is made up of glycoproteins such as. To investigate how substrate properties influence stem cell fate, we cultured single human epidermal stem cells on polydimethylsiloxane pdms and polyacrylamide paam hydrogel surfaces, 0. Ecm is an essential component of the stem cell niche, and it is. Extracellular matrix as a regulator of epidermal stem cell fate. Extracellular matrix tethering regulates stem cell fate. The recent literature has reported many studies focused on the. The extracellular matrix ecm, once thought to function only as a scaffold to maintain tissue and organ structure, regulates many aspects of cell behavior, including cell proliferation and growth, survival, change in cell shape, migration, and differentiation.
This interaction is dynamic, multidimensional and reciprocally evolving through time, and from this concerted exchange the. Stemcell fate is known to be regulated by signals from the microenvironment, one of the. Changes in cellular state can be regulated by mechanical signals from the cellular microenvironment, such as the local extracellular matrix ecm stiffness 1,2,3,4. Moreover, the niche dynamically regulates stem cell behavior, maintaining a balance between quiescence, selfrenewal and differentiation 20, 21. Stem cell behaviors are regulated by multiple microenvironmental cues. In this work, we clarify how surface energy of soft biomaterials can dramatically affect.
The stem cell fate is regulated by several factors. Mechanism of regulation of stem cell differentiation by. The extracellular matrix ecm regulates cell behavior by influencing cell proliferation, survival, shape, migration and differentiation. Basically only animal cells have ecm or extracellular matrix. Each cell forever interacts with its extracellular matrix ecm. Jul 07, 2016 the extracellular matrix ecm is one such component involved in mediating stem cell fate. The mechanical properties of the extracellular matrix ecm in which cells reside have emerged as an important regulator of cell fate. The stiffness, nanotopography, protein composition, stress and strain inherent to any given ecm influences stem cell lineage commitment. The design and development of biomaterials mimicking the natural environment of different cell types, the socalled extracellular matrix ecm, is the focus of research in this field. Although several studies have been done to understand the conditions that promote proliferation, differentiation and. Their components change over time, reflecting diverse functions in environment sensing. To investigate how substrate properties influence stemcell fate, we cultured single human epidermal stem cells on polydimethylsiloxane pdms and polyacrylamide paam hydrogel surfaces, 0.
They could be used for surveying the usage of biological small molecules during differentiation and. While materials based on natural ecm have been used to implicate the role of substrate stiffness for cell fate decisions, it is difficult in these matrices to isolate mechanics from other structural parameters. Discovering celladhesion peptides in tissue engineering. Mechanical influence of tissue culture plates and extracellular matrix. Their components change over time, reflecting diverse functions in environment sensing, adhesion, and cell fate determination. Extracellular matrix proteins are commonly used in cell culture systems to maintain stem and precursor cells in an undifferentiated state during cell culture and function to induce differentiation of epithelial, endothelial and smooth muscle cells in vitro. There is no one matrix though, with different tissues having their own.
Stem cells reside within most tissues throughout the lifetimes of mammalian organisms. Sorry, we are unable to provide the full text but you may find it at the following locations. Extracellularmatrix tethering regulates stemcell fate article pdf available in nature materials 117. Stem cell fate is known to be regulated by signals from the microenvironment, one of the environmental parameters being the extracellular matrix ecm to which stem cells adhere 1. However, due to poor cell survival, proliferation and differentiation in the patient, the therapy outcomes have not been satisfactory. Mesenchymal stem cell msc fate is influenced by past mechanical dosing memory, but the mechanisms underlying this process have not yet been well defined. Understanding the extracellular forces that determine cell fate and. Mesenchymal stem cells msc can differentiate into several cell types and are desirable candidates for cell therapy and tissue engineering. Stem cells regulate their fate by binding to, and contracting against, the extracellular matrix. A key challenge in stem cell research is to learn how to direct the differentiation of stem cells toward specific fates. Extracellularmatrix tethering regulates stem cell fate. Stem cells reside in a dynamic and specialized microenvironment denoted as niche. Extracellular matrix tethering regulates stem cell fate britta trappmann 1, julien e.
In particular, new data continue to mount on the influence of the extracellular matrix ecm on stem cell fate through physical interactions with cells, such as the control of cell geometry, ecm geometrytopography at the nanoscale, ecm mechanical properties, and the transmission of mechanical or. May 27, 2012 extracellular matrix tethering regulates stem cell fate. Jul 24, 2012 in the version of this article originally published, in fig. Extracellularmatrix tethering regulates stemcell fate britta trappmann 1, julien e. This cited by count includes citations to the following articles in scholar.
These signals may be structural, physical, electrical, or biochemical. Review biophysical regulation of stem cell behavior within. To facilitate metabolomics analysis of stem cell differentiation, alakpa et al. Extracellularmatrix tethering regulates stemcell fate nature. Extracellular matrix type modulates mechanotransduction of. The extracellular matrix ecm is one such component involved in mediating stem cell. A mathematical model of mechanotransduction reveals how.
We here reveal that the choice of ecm type can directly modulate stem cell mechanotransduction, filling this critical gap. Biomaterial surface energydriven ligand assembly strongly. Recently, it has been proposed that in addition to. May 01, 2018 cell instructive biomaterial cues are a major topic of interest in both basic and applied research. The mechanical properties of the extracellular matrix ecm in. The extracellular microscape governs mesenchymal stem cell fate.
We have yet to understand how memory affects specific cell fate decisions, such as the differentiation of mscs into neurons, adipocytes, myocytes, and osteoblasts. These gels facilitate stiffnesstuned stem cell differentiation without having to rely on biochemical functionalization. However, only a handful of caps have been utilized. Basically only animal cells have ecm or extracellular matrix, because plants have their tough cell walls that support and protect them. May 27, 2015 stem cell behaviors are regulated by multiple microenvironmental cues. Recent studies have made significant progress in understanding stem cellecm interactions. Collagen promotes higher adhesion, survival and proliferation. The stem cell fate is regulated by several factors, such as growth factors or transcription. Extracellularmatrix tethering regulates stem cell fate britta trappmann 1, julien e. Interplay of matrix stiffness and protein tethering in. In vivo stem cells live in a complex microenvironment called the stem cell niche. Control of stem cell fate by physical interactions with. May 27, 2012 stem cell fate is known to be regulated by signals from the microenvironment, one of the environmental parameters being the extracellular matrix ecm to which stem cells adhere 1.
Cell instructive biomaterial cues are a major topic of interest in both basic and applied research. Bioengineered niches can be used for investigating stem cellmatrix interactions. As an alternative to natural extracellular matrix ecm macromolecules, cell adhesion peptides caps have had tremendous impact on the design of cell culture platforms, implants, and wound dressings. One of those signals is from the extracellular matrix ecm. How varying the type of ecm modulates stem cell mechanotransduction remains largely unknown. Effects of matrix stiffness on the morphology, adhesion. The mechanical properties of the extracellular matrix ecm in which cells reside have. In the version of this article originally published, in fig. Nov 26, 2015 the development of biomaterials for tissue engineering applications is continuously improving, accordingly to the needs to generate an ideal cellextracellular matrix interaction. By changing the stiffness of the substrate, human mesenchymal stem cells could be directed along neuronal, muscle, or bone lineages. Pdf role of the extracellular matrix in regulating stem cell fate. As an external signal, mechanical stiffness of the extracellular matrix is capable of governing stem cell fate determination, but how this biophysical cue is translated into intracellular signaling remains elusive.
Electronic publications 80689 freely accessible full text publications plus those not yet available due to embargo. Mechanical influence of tissue culture plates and extracellular matrix on mesenchymal stem cell behavior. We also discuss how external factors in the mammary gland microenvironment, such as extracellular matrix ecm and cell cell interactions, influence cell fate and function. The predominantly perinuclear mitochondrial distribution on col suggests that col matrix might maintain msc in more stem cell like state. The 2012 annual meeting of the international society for stem cell research isscr marked the tenth anniversary of the isscr. As it was shown in multiple studies, extracellular matrix ecm contributes a lot to stem cell niches in various tissues, including that of skin. To maintain their capacities for division and differentiation and thereby build, maintain, and regenerate. Epidermal stem cells reside within the specific anatomic location, called niche, which is a microenvironment that interacts with stem cells to regulate their fate. Start studying extracellular matrix and cell junctions.
We elucidate how surface protein selfassembly and the resulting surface topology can act to steer. Far from being a static structure, the ecm is constantly. Biophysical regulation of stem cell behavior within the. Interplay of matrix stiffness and protein tethering in stem cell. Extracellular matrix regulation of stem cell behavior. In particular, new data continue to mount on the influence of the extracellular matrix ecm on stem cell. Mesenchymal stem cells mscs are controlled by signals from the ecm. Dynamic bioengineered hydrogels as scaffolds for advanced stem cell and organoid culture volume 7 issue 3 laura c. The stem cell niche influences stem cell behavior and regulates stem cell fate decisions by providing a variety of signals. This study aimed to determine how matrix stiffness affects cell morphology and subsequently regulates the osteogenic phenotype of osteogenesis precursor cells.
In this work, we clarify how surface energy of soft biomaterials can dramatically affect mesenchymal stem cell receptor recruitment and downstream signaling related to cell fate. A diverse array of environmental factors contributes to the overall control of stem cell activity. Supplementary information for extracellularmatrix tethering regulates stemcell fate britta trappmann, julien e. Recent studies into mechanotransduction have demonstrated that cells sense and integrate mechanical cues from the ecm, causing transcriptional. The extracellular matrix represents an essential component of stem cell niches. Stem cell behavior is altered by the mechanical and biochemical properties of the surrounding extracellular matrix ecm. Pdf the field of stem cells and regenerative medicine offers considerable promise as a means of delivering new. Extracellular matrix and integrins in embryonic stem cell. The stiffness, nanotopography, protein composition, stress and.
Pdf extracellularmatrix tethering regulates stemcell. Recent studies into mechanotransduction have demonstrated that cells sense and integrate mechanical cues from the ecm, causing transcriptional changes to occur and influencing cell fate decisions, 5. As an external signal, mechanical stiffness of the extracellular matrix is capable of governing stem cell fate. The extracellular microscape governs mesenchymal stem cell fate william j. Four pdms substrates were prepared with stiffness corresponding to the. Extracellular matrix tethering regulates stem cell fate by britta trappmann, julien e gautrot, john t connelly, daniel g t strange, yuan li, michelle l oyen, martien a cohen stuart, heike boehm, bojun li, viola vogel, joachim p spatz, fiona m watt and wilhelm t s huck. Recently, it has been proposed that in addition to matrix stiffness and ligand type, the degree of coupling of. The extracellular matrix is a critical regulator of stem cell function. Extracellularmatrix tethering regulates stem cell fate article pdf available in nature materials 117. Control of stem cell fate by physical interactions with the. Dynamic bioengineered hydrogels as scaffolds for advanced. Guilak f, cohen dm, estes bt, gimble jm, liedtke w, chen cs. Extracellular matrix dynamics in development and regenerative.
Regulation of many important processes, including maintenance of stem cell quiescence, selfrenewal, and homeostasis, as well as the regulation of division and differentiation, are common functions of the stem cell niche. Selfassembling peptides as extracellular matrix mimics to. Pdf extracellularmatrix tethering regulates stemcell fate. Pdf to investigate how substrate properties influence stemcell fate, we cultured single human epidermal stem cells on polydimethylsiloxane. However, extracellular matrix ecm is comprised of different protein types. Extracellularmatrix tethering regulates stemcell fate core. Control of stem cell fate by physical interactions with the extracellular matrix. Osteogenesisrelated behavior of mc3t3e1 cells on substrates. Trappmann b1, gautrot je, connelly jt, strange dg, li y, oyen ml, cohen stuart ma, boehm h, li b, vogel v, spatz jp, watt fm. Recently, it has been proposed that in addition to matrix sti. Interest in biologically active materials that can be used as cell culture substrates for medicinal applications has increased dramatically over the last decade.
Here, we elucidate mechanisms by which stem cells respond to microenvironmental stiffness through the dynamics. Emphasis is placed on models using natural and synthetic biomaterials to generate scaffolds mimicking the extracellular matrix, which is known to play a critical role in angiogenesis. The extracellular matrix ecm is one such component involved in mediating stem cell fate. The discrepancy in ecm composition strongly affects cell behavior, so it is paramount to reproduce such differences in synthetic systems. Mar 27, 2019 interest in biologically active materials that can be used as cell culture substrates for medicinal applications has increased dramatically over the last decade. Biomaterials mimic passive and active cues that regulate stem cell responses. Ecm support cell adhesion, among many other functions, and. Osteogenic differentiation of cells has considerable clinical significance in bone defect treatment, and cell behavior is linked to extracellular matrix stiffness.
1065 106 968 1555 768 1052 362 856 509 1192 1616 1549 479 1364 1283 958 617 624 1443 601 356 846 1180 1578 1197 715 606 716 369 117 852 98 843 1332 1223 1012 170 408 73 21 531 1240